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KMID : 0380020170320020096
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Korean Journal of Biotechnology and Bioengineering
2017 Volume.32 No. 2 p.96 ~ p.102
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Solid State Fermentation of Phanerochaete chrysosporium for Degradation and Saccharification of Lignocellulose
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Utomo Romualdus N. C.
Lee Eun-Kwang
Yun Hyun-Shik
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Abstract
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The lignocellulose that is a major component of spent coffee ground was degraded and saccharified. To implement the spent coffee, after several pre-treatments, inoculation of Phanerochaete chrysosporium and solid-state fermentation were conducted. The optimal temperature of the enzymes (lignin peroxidase, manganese peroxidase, xylanase, laccase, and cellulase) for degradation of lignocellulose by P. chrysosporium was found. We also measured the maximum activity of enzymes (lignin peroxidase 0.15 IU/mL, manganese peroxidase 0.90 IU/mL, laccase 0.11 IU/mL, cellulase 5.87 IU/mL, carboxymethyl cellulase 9.52 IU/mL, xylanase 1.16 IU/mL) used for the process. As a result, 4.73 mg/mL of reduced sugar was obtained and 61.02% of lignin was degraded by solid state fermentation of P. chrysosporium on spent coffee medium.
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KEYWORD
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Biomass, Spent coffee, Lignocellulose degradation, Lignin degradation, Phanerochaete chrysosporium
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